Impact of glucose and propionic acid on even and odd chain fatty acid profiles of oleaginous yeasts
Artikel i vetenskaplig tidskrift, 2025

Background: Odd chain fatty acids (OCFAs) are gaining attention for their valuable medical and nutritional applications. Microbial fermentation offers a sustainable and environmentally friendly alternative for OCFA production compared to traditional extraction or chemical synthesis methods. To achieve an economically feasible OCFA production process, it is essential to identify and develop microbial cell factories capable of producing OCFAs with high titers and yields. Results: We selected 19 yeast species, including both oleaginous yeasts and representatives from the Ascomycota and Basidiomycota phyla, based on their known or potential ability to produce OCFAs. These species were screened under various growth conditions to evaluate their OCFA production potential. In glucose-based, nitrogen-limited media, the strains produced fatty acids to varying extents, with OCFAs comprising 0.5–5% of the total fatty acids. When using the OCFAs precursor propionic acid as the sole carbon source, only eight strains exhibited growth, with tolerance to propionic acid concentrations between 5 and 29 g/L. The strains also displayed varying efficiencies in converting propionic acid into fatty acids, yielding between 0.16 and 1.22 g/L of fatty acids, with OCFAs constituting 37–89% of total fatty acids. Among the top performing strains, Cutaneotrichosporon oleaginosus produced the highest OCFA titers and yields (0.94 g/L, 0.07 g/g), Yarrowia lipolytica demonstrated superior growth rates even at elevated propionic acid concentrations, and Rhodotorula toruloides achieved the highest proportion of OCFAs relative to total fatty acids (89%). Conclusions: Our findings highlight the diverse capacities of the selected yeast species for OCFA production, identifying several promising strains for further optimization as microbial cell factories in sustainable OCFA production processes.

Short chain fatty acid (SCFA)

Cutaneotrichosporon oleaginosus

Propionic acid tolerance

Rhodotorula toruloides

Yarrowia lipolytica

Odd chain fatty acid (OCFA)

Fatty acids methyl ester

Non-conventional yeast

Even chain fatty acid (ECFA)

Författare

Veronica Bonzanini

Chalmers, Life sciences, Industriell bioteknik

AAK AB

Majid Haddad Momeni

AAK AB

Kim Olofsson

AAK AB

Lisbeth Olsson

Chalmers, Life sciences, Industriell bioteknik

Cecilia Geijer

Chalmers, Life sciences, Industriell bioteknik

BMC Microbiology

14712180 (eISSN)

Vol. 25 1 79

Ämneskategorier (SSIF 2025)

Molekylärbiologi

Annan biologi

Cellbiologi

Mikrobiologi

Infrastruktur

Chalmers infrastruktur för masspektrometri

Styrkeområden

Energi

Hälsa och teknik

DOI

10.1186/s12866-025-03788-w

PubMed

39966733

Mer information

Senast uppdaterat

2025-04-07