Probing physical properties of DNA-protein complexes using nanofluidic channels
Paper i proceeding, 2013

We present the use of nanofluidic channels as a tool for determining physical properties of single DNA-protein complexes. By coating the nanochannels with a lipid bilayer we avoid sticking of proteins to the channel walls. RecA is a prokaryotic protein involved in recombination and DNA repair. We study filaments of RecA, bound to both double stranded (ds) and single stranded (ss) DNA. We determine the persistence length of RecA filaments on both dsDNA and ssDNA and obtain values in agreement with the literature. Neither the DNA nor the protein has to be attached to handles or surfaces, and the technique is directly transferable to Lab-on-a-Chip technologies for high throughput measurements in solution.

DNA-protein interactions

Single DNA molecules

Lab-on-a-Chip

Nanofluidics

Författare

Karolin Frykholm

Kemisk biologi

Mohammadreza Alizadehheidari

Kemisk biologi

Louise Fornander

Fysikalisk kemi

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

Jens Wigenius

Fysikalisk kemi

P. J. Beuning

Northeastern University

M. Modesti

Aix-Marseille Université

F. Persson

Uppsala universitet

Fredrik Westerlund

Fysikalisk kemi

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 2 1311-1313
978-163266624-6 (ISBN)

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)

Ämneskategorier

Fysikalisk kemi

Nanoteknik

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Senast uppdaterat

2019-11-26