Flux regulation through glycolysis and respiration is balanced by inositol pyrophosphates in yeast
Journal article, 2023

Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid-glycolysis yeast. Subsequently, we identified an inositol pyrophosphatase encoded by OCA5 that could regulate glycolysis and respiration by adjusting 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP7) levels. 5-InsP7 levels could regulate the expression of genes involved in glycolysis and respiration, representing a global mechanism that could sense ATP levels and regulate central carbon metabolism. The hybrid-glycolysis yeast did not produce ethanol during growth under excess glucose and could produce 2.68 g/L free fatty acids, which is the highest reported production in shake flask of Saccharomyces cerevisiae. This study demonstrated the significance of hybrid-glycolysis yeast and determined Oca5 as an inositol pyrophosphatase controlling the balance between glycolysis and respiration, which may shed light on the role of inositol pyrophosphates in regulating eukaryotic metabolism.

Mig1/Hap4 signaling pathway

free fatty acids

OCA5

hybrid-glycolysis yeast

inositol pyrophosphate

Author

Ning Qin

Beijing University of Chemical Technology

Chalmers, Life Sciences, Systems and Synthetic Biology

Lingyun Li

Chalmers, Life Sciences, Systems and Synthetic Biology

Beijing University of Chemical Technology

Xu Ji

Beijing University of Chemical Technology

Rui Pereira

Chalmers, Life Sciences, Systems and Synthetic Biology

Yu Chen

Chalmers, Life Sciences, Systems and Synthetic Biology

Shile Yin

Beijing University of Chemical Technology

Chaokun Li

University of Helsinki

Xiaozhen Wan

Beijing University of Chemical Technology

Danye Qiu

University of Freiburg

Junfeng Jiang

Tianjin Institute of Industrial Biotechnology

Hao Luo

Chalmers, Life Sciences, Systems and Synthetic Biology

Yueping Zhang

China Agricultural University

Genlai Dong

Beijing University of Chemical Technology

Yiming Zhang

Beijing University of Chemical Technology

Chalmers, Life Sciences, Systems and Synthetic Biology

Shuobo Shi

System Biology

Beijing University of Chemical Technology

Henning J. Jessen

University of Freiburg

Jianye Xia

Tianjin Institute of Industrial Biotechnology

Yun Chen

Chalmers, Life Sciences, Systems and Synthetic Biology

Christer Larsson

Chalmers, Life Sciences, Systems and Synthetic Biology

Tianwei Tan

Beijing University of Chemical Technology

Zihe Liu

Beijing University of Chemical Technology

System Biology

Jens B Nielsen

Beijing University of Chemical Technology

Chalmers, Life Sciences, Systems and Synthetic Biology

BioInnovation Institute

Cell

0092-8674 (ISSN) 1097-4172 (eISSN)

Vol. 186 4 748-763.e15

Subject Categories

Cell Biology

Microbiology

Plant Biotechnology

DOI

10.1016/j.cell.2023.01.014

PubMed

36758548

More information

Latest update

3/3/2023 9