Flux regulation through glycolysis and respiration is balanced by inositol pyrophosphates in yeast
Journal article, 2023
Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid-glycolysis yeast. Subsequently, we identified an inositol pyrophosphatase encoded by OCA5 that could regulate glycolysis and respiration by adjusting 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP7) levels. 5-InsP7 levels could regulate the expression of genes involved in glycolysis and respiration, representing a global mechanism that could sense ATP levels and regulate central carbon metabolism. The hybrid-glycolysis yeast did not produce ethanol during growth under excess glucose and could produce 2.68 g/L free fatty acids, which is the highest reported production in shake flask of Saccharomyces cerevisiae. This study demonstrated the significance of hybrid-glycolysis yeast and determined Oca5 as an inositol pyrophosphatase controlling the balance between glycolysis and respiration, which may shed light on the role of inositol pyrophosphates in regulating eukaryotic metabolism.
OCA5
hybrid-glycolysis yeast
Mig1/Hap4 signaling pathway
free fatty acids
inositol pyrophosphate
Author
Ning Qin
Chalmers, Life Sciences, Systems and Synthetic Biology
Beijing University of Chemical Technology
Lingyun Li
Chalmers, Life Sciences, Systems and Synthetic Biology
Beijing University of Chemical Technology
Xu Ji
Beijing University of Chemical Technology
Rui Pereira
Chalmers, Life Sciences, Systems and Synthetic Biology
Yu Chen
Chalmers, Life Sciences, Systems and Synthetic Biology
Shile Yin
Beijing University of Chemical Technology
Chaokun Li
University of Helsinki
Xiaozhen Wan
Beijing University of Chemical Technology
Danye Qiu
University of Freiburg
Junfeng Jiang
Chinese Academy of Sciences
Hao Luo
Chalmers, Life Sciences, Systems and Synthetic Biology
Yueping Zhang
China Agricultural University
Genlai Dong
Beijing University of Chemical Technology
Yiming Zhang
Beijing University of Chemical Technology
Chalmers, Life Sciences, Systems and Synthetic Biology
Shuobo Shi
System Biology
Beijing University of Chemical Technology
Henning J. Jessen
University of Freiburg
Jianye Xia
Chinese Academy of Sciences
Yun Chen
Chalmers, Life Sciences, Systems and Synthetic Biology
Christer Larsson
Chalmers, Life Sciences, Systems and Synthetic Biology
Tianwei Tan
Beijing University of Chemical Technology
Zihe Liu
System Biology
Beijing University of Chemical Technology
Jens B Nielsen
Beijing University of Chemical Technology
BioInnovation Institute
Chalmers, Life Sciences, Systems and Synthetic Biology
Cell
0092-8674 (ISSN) 1097-4172 (eISSN)
Vol. 186 4 748-763.e15Subject Categories (SSIF 2011)
Cell Biology
Microbiology
Plant Biotechnology
DOI
10.1016/j.cell.2023.01.014
PubMed
36758548