Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
Journal article, 2017

Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l(-1) in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.

arabidopsis

jojoba oil

reductases

synthase

acetyl-coenzyme

yeast

elongation

Science & Technology - Other Topics

genetic modifications

biosynthesis

pathway

Author

Tao Yu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Yongjin Zhou

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Leonie Wenning

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Quanli Liu

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Anastasia Krivoruchko

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Florian David

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Nature Communications

2041-1723 (ISSN)

Vol. 8 Article number: 15587- 15587

Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds (CHASSY)

European Commission (Horizon 2020), 2016-12-01 -- 2020-11-30.

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Biochemistry and Molecular Biology

Microbiology

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1038/ncomms15587

More information

Latest update

11/7/2018