Functionalized Nanostructures: Redox-Active Porphyrin Anchors for Supramolecular DNA Assemblies
Journal article, 2010
We have synthesized and studied a supramolecular system comprising a 39-mer DNA with porphyrin-modified thymidine nucleosides anchored to the surface of large unilamellar vesicles (liposomes). Liposome porphyrin binding characteristics, such as orientation, strength, homogeneity, and binding site size, was determined, suggesting that the porphyrin is well suited as a photophysical and redox-active lipid anchor, in comparison to the inert cholesterol anchor commonly used today. Furthermore, the binding characteristics and hybridization capabilities were studied as a function of anchor size and number of anchoring points, properties that are of importance for our future plans to use the addressability of these redox-active nodes in larger DNA-based nanoconstructs. Electron transfer from photoexcited porphyrin to a lipophilic benzoquinone residing in the lipid membrane was characterized by steady-state and time-resolved fluorescence and verified by femtosecond transient absorption.
electron
dependence
transfer
membrane
porphyrin
supramolecular
DNA
nanotechnology
construction
scaffold
bridge-acceptor systems
flow
orientation
fluorescence
dichroism
liposomes
membrane
photoinduced electron-transfer
Author
Karl Börjesson
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Joanna Wiberg
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Afaf El-Sagheer
University of Southampton
Suez Canal University
Thomas Ljungdahl
University of Gothenburg
Jerker Mårtensson
Chalmers, Chemical and Biological Engineering, Organic Chemistry
T. Brown
University of Southampton
Bengt Nordén
Chalmers, Chemical and Biological Engineering, Physical Chemistry
Bo Albinsson
Chalmers, Chemical and Biological Engineering, Physical Chemistry
ACS Nano
1936-0851 (ISSN) 1936-086X (eISSN)
Vol. 4 9 5037-5046Areas of Advance
Nanoscience and Nanotechnology
Energy
Life Science Engineering (2010-2018)
Materials Science
Subject Categories
Chemical Sciences
DOI
10.1021/nn100667b