A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications
Artikel i vetenskaplig tidskrift, 2012

The properties of a cell's microenvironment are one of the main driving forces in cellular fate processes and phenotype expression in vivo. The ability to create controlled cell microenvironments in vitro becomes increasingly important for studying or controlling phenotype expression in tissue engineering and drug discovery applications. This includes the capability to modify material surface properties within well-defined liquid environments in cell culture systems. One successful approach to mimic extra cellular matrix is with porous electrospun polymer fiber scaffolds, while microfluidic networks have been shown to efficiently generate spatially and temporally defined liquid microenvironments. Here, a method to integrate electrospun fibers with microfluidic networks was developed in order to form complex cell microenvironments with the capability to vary relevant parameters. Spatially defined regions of electrospun fibers of both aligned and random orientation were patterned on glass substrates that were irreversibly bonded to microfluidic networks produced in poly-dimethyl-siloxane. Concentration gradients obtained in the fiber containing channels were characterized experimentally and compared with values obtained by computational fluid dynamic simulations. Velocity and shear stress profiles, as well as vortex formation, were calculated to evaluate the influence of fiber pads on fluidic properties. The suitability of the system to support cell attachment and growth was demonstrated with a fibroblast cell line. The potential of the platform was further verified by a functional investigation of neural stem cell alignment in response to orientation of electrospun fibers versus a microfluidic generated chemoattractant gradient of stromal cell-derived factor 1 alpha. The described method is a competitive strategy to create complex microenvironments in vitro that allow detailed studies on the interplay of topography, substrate surface properties, and soluble microenvironment on cellular fate processes.

bioMEMS

stem-cells

growth

breast-cancer cells

diameter

drugs

in-vitro

adhesion

biomedical equipment

gradients

nanofibers

cellular biophysics

computational fluid dynamics

strength

migration

Författare

Patric Wallin

Chalmers, Teknisk fysik, Biologisk fysik

Carl Zandén

Chalmers, Mikroteknologi och nanovetenskap (MC2), Elektronikmaterial och system

Björn Carlberg

Chalmers, Mikroteknologi och nanovetenskap (MC2), Elektronikmaterial och system

Nina Hellström Erkenstam

Göteborgs universitet

Johan Liu

Chalmers, Mikroteknologi och nanovetenskap (MC2), Elektronikmaterial och system

Julie Gold

Chalmers, Teknisk fysik, Biologisk fysik

Biomicrofluidics

1932-1058 (ISSN)

Vol. 6 2 024131

Ämneskategorier

Fysik

DOI

10.1063/1.4729747