Splenic Immune Response Is Down-Regulated in C57BL/6J Mice Fed Eicosapentaenoic Acid and Docosahexaenoic Acid Enriched High Fat Diet
Artikel i vetenskaplig tidskrift, 2017

© 2017 by the authors; licensee MDPI, Basel, Switzerland. Dietary n-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are associated with reduction of inflammation, although the mechanisms are poorly understood, especially how the spleen, as a secondary lymphoid organ, is involved. To investigate the effects of EPA and DHA on spleen gene expression, male C57BL/6J mice were fed high fat diets (HFD) differing in fatty acid composition, either based on corn oil (HFD-CO), or CO enriched with 2 g/100 g EPA and DHA (HFD-ED), for eight weeks. Spleen tissue was analyzed using transcriptomics and for fatty acids profiling. Biological processes (BPs) related to the immune response, including T-cell receptor signaling pathway, T-cell differentiation and co-stimulation, myeloid dendritic cell differentiation, antigen presentation and processing, and the toll like receptor pathway were downregulated by HFD-ED compared with control and HFD-CO. These findings were supported by the down-regulation of NF-κB in HFD-ED compared with HFD-CO fed mice. Lower phospholipid arachidonic acid levels in HFD-ED compared with HFD-CO, and control mice suggest attenuation of pathways via prostaglandins and leukotrienes. The HFD-ED also upregulated BPs related to erythropoiesis and hematopoiesis compared with control and HFD-CO fed mice. Our findings suggest that EPA and DHA down-regulate the splenic immune response induced by HFD-CO, supporting earlier work that the spleen is a target organ for the anti-inflammatory effects of these n-3 fatty acids.


Nikulkumar Soni

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Alastair Ross

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Nathalie Scheers

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Otto Savolainen

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Intawat Nookaew

Chalmers, Biologi och bioteknik, Systembiologi

Britt Gabrielsson

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ann-Sofie Sandberg

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap


2072-6643 (ISSN) 20726643 (eISSN)

Vol. 9 1 50- 50


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Data- och informationsvetenskap

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Chalmers infrastruktur för masspektrometri


Livsvetenskaper och teknik (2010-2018)



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