Understanding enzyme-substrate interactions in Carbohydrate Esterase Family 15
Poster (konferens), 2019
An in-depth understanding of how CE15 enzymes interact with their complex substrates is still lacking, as only one structure with a monosaccharide ligand has been solved to date [3]. To address this, we have pursued solving new GE structures and obtaining protein-ligand complex structures. The studies have resulted in a novel structure exhibiting features with prominent inserts surrounding the active site, suggesting different specificities between bacterial and fungal GEs. In addition, we have solved the first structures of a CE15 enzyme with larger ligands, which gives direct evidence of how these enzymes interact with the different parts of its proposed physiological LCC substrates. Combined with kinetic characterizations, these new investigations greatly add to the knowledge of enzyme-substrate interactions in CE15 and enhances how these enzymes may act in natural conditions, which could aid in industrial biomass conversion.
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[1] Španiková S.; Biely P. FEBS Lett. 2006, 580, 4597-4601.
[2] Arnling Bååth J.; Mazurkewich S.; Knudsen R.M.; Poulsen J.N.; Olsson L.; Lo Leggio L.; Larsbrink J. Biotechnol Biofuels. 2018, 11, 213-226.
[3] Charavgi M.D.; Dimarogona M.; Topakas E.; Christakopoulos P.; Chrysina E.D. Acta Crystallogr. D Biol. Crystallogr. 2013, 69, 63-73.
lignin-carbohydrate complexes
glucuronoyl esterase
biochemistry
CE15
crystallography
structural biology
glucuronoxylan
Författare
Scott Mazurkewich
Chalmers, Biologi och bioteknik, Industriell bioteknik
Jenny Arnling Bååth
Chalmers, Biologi och bioteknik, Industriell bioteknik
Jens-Christian N Poulsen
Köpenhamns universitet
Lisbeth Olsson
Chalmers, Biologi och bioteknik, Industriell bioteknik
Leila Lo Leggio
Köpenhamns universitet
Johan Larsbrink
Chalmers, Biologi och bioteknik, Industriell bioteknik
Toulouse, France,
Structure-based engineering of glucuronoyl esterases for separation of lignin and carbohydrates
Novo Nordisk Fonden (27698), 2018-01-01 -- 2020-12-31.
Ämneskategorier
Biokemi och molekylärbiologi
Strukturbiologi
Biokatalys och enzymteknik
Fundament
Grundläggande vetenskaper
Styrkeområden
Livsvetenskaper och teknik (2010-2018)