Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
Paper i proceeding, 2019

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Nanofluidics

Optical DNA Mapping

Bacterial Identification

Författare

My Nyblom

Chalmers, Biologi och bioteknik, Kemisk biologi

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

Anna Johnning

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Göteborgs universitet

Stiftelsen Fraunhofer-Chalmers Centrum för Industrimatematik

Marie Wrande

Uppsala universitet

Albertas Dvirnas

Lunds universitet

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Christian G. Giske

Karolinska Institutet

Karolinska universitetssjukhuset

Tobias Ambjörnsson

Lunds universitet

L. Sandegren

Uppsala universitet

Erik Kristiansson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

821-822

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

Mer information

Senast uppdaterat

2020-11-13