Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

My Nyblom; Vilhelm Müller; Anna Johnning; Marie Wrande; Albertas Dvirnas; Sriram Kesarimangalam; Christian G. Giske; Tobias Ambjörnsson; L. Sandegren; Erik Kristiansson; Fredrik Westerlund

Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
Paper i proceeding, 2019

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Nanofluidics

Optical DNA Mapping

Bacterial Identification

Författare

My Nyblom

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Anna Johnning

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Göteborgs universitet

Stiftelsen Fraunhofer-Chalmers Centrum för Industrimatematik

Forskning Andra publikationer

Marie Wrande

Uppsala universitet

Albertas Dvirnas

Lunds universitet

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Christian G. Giske

Karolinska Institutet

Karolinska universitetssjukhuset

Tobias Ambjörnsson

Lunds universitet

Forskning Andra publikationer

L. Sandegren

Uppsala universitet

Erik Kristiansson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Forskning Andra publikationer

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

821-822
9781733419000 (ISBN)

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

Mer information

Senast uppdaterat

2020-11-13

Bacterial identification by optical mapping of genomic DNA in nanofluidic channels Paper i proceeding, 2019

Författare

My Nyblom

Vilhelm Müller

Anna Johnning

Marie Wrande

Albertas Dvirnas

Sriram Kesarimangalam

Christian G. Giske

Tobias Ambjörnsson

L. Sandegren

Erik Kristiansson

Fredrik Westerlund

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Ämneskategorier

Mer information

Senast uppdaterat

Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
Paper i proceeding, 2019