Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
Paper in proceedings, 2019

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Nanofluidics

Optical DNA Mapping

Bacterial Identification

Author

My Nyblom

Chalmers, Biology and Biological Engineering, Chemical Biology

Vilhelm Müller

Chalmers, Biology and Biological Engineering, Chemical Biology

Anna Johnning

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

University of Gothenburg

Fraunhofer-Chalmers Centre

Marie Wrande

Uppsala University

Albertas Dvirnas

Lund University

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Christian G. Giske

Karolinska Institutet

Karolinska University Hospital

Tobias Ambjörnsson

Lund University

L. Sandegren

Uppsala University

Erik Kristiansson

University of Gothenburg

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

821-822

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Subject Categories

Infectious Medicine

Microbiology

Microbiology in the medical area

More information

Latest update

11/13/2020