Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance
Artikel i vetenskaplig tidskrift, 2022

The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscopic liposomes especially with respect to the level of deformation induced upon their ligand-receptor-mediated interaction with laterally fluid cellular membranes. Here, we utilize the sensitivity of dual-wavelength surface plasmon resonance to probe the size and shape of bound liposomes (∼100 nm in diameter) as a means to quantify receptor-induced deformation during their interaction with a supported cell membrane mimic. By comparing biotinylated liposomes in gel and fluid phases, we demonstrate that fluid-phase liposomes are more prone to deformation than their gel-phase counterparts upon binding to the cell membrane mimic and that, as expected, the degree of deformation depends on the number of ligand-receptor pairs that are engaged in the multivalent binding.

Författare

Karin Norling

Chalmers, Biologi och bioteknik, Kemisk biologi

Mattias Sjöberg

Chalmers, Fysik, Nano- och biofysik

Marta Bally

Umeå universitet

Vladimir Zhdanov

Russian Academy of Sciences

Chalmers, Fysik

Nagma Parveen

Chalmers, Fysik, Biologisk fysik

Fredrik Höök

Chalmers, Fysik, Nano- och biofysik

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 38 8 2550-2560

Tvådimensionell flödescytometry för analys av enskilda nanopartiklar

Vetenskapsrådet (VR) (2018-04900), 2018-12-01 -- 2021-12-31.

Funktionell leverans av nukleotid-baserade läkemedel

Stiftelsen för Strategisk forskning (SSF) (IRC15-0065), 2017-03-01 -- 2024-12-31.

Ämneskategorier

Biokemi och molekylärbiologi

Annan medicinsk grundvetenskap

Biofysik

DOI

10.1021/acs.langmuir.1c03096

PubMed

35156833

Mer information

Senast uppdaterat

2022-03-03