Eicosapentaenoic and Docosahexaenoic Acid-Enriched High Fat Diet Delays Skeletal Muscle Degradation in Mice
Artikel i vetenskaplig tidskrift, 2016

Low-grade chronic inflammatory conditions such as ageing, obesity and related metabolic disorders are associated with deterioration of skeletal muscle (SkM). Human studies have shown that marine fatty acids influence SkM function, though the underlying mechanisms of action are unknown. As a model of diet-induced obesity, we fed C57BL/6J mice either a high fat diet (HFD) with purified marine fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (HFD-ED), a HFD with corn oil, or normal mouse chow for 8 weeks; and used transcriptomics to identify the molecular effects of EPA and DHA on SkM. Consumption of ED-enriched HFD modulated SkM metabolism through increased gene expression of mitochondrial β-oxidation and slow-fiber type genes compared with HFD-corn oil fed mice. Furthermore, HFD-ED intake increased nuclear localization of nuclear factor of activated T-cells (Nfatc4) protein, which controls fiber-type composition. This data suggests a role for EPA and DHA in mitigating some of the molecular responses due to a HFD in SkM. Overall, the results suggest that increased consumption of the marine fatty acids EPA and DHA may aid in the prevention of molecular processes that lead to muscle deterioration commonly associated with obesity-induced low-grade inflammation.

obesity

skeletal-muscle metabolism

transcriptome

mitochondrial β-oxidation

eicosapentaenoic acid (EPA)/Docosahexaenoic acid (DHA)

Författare

Nikulkumar Soni

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Alastair Ross

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Nathalie Scheers

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Otto Savolainen

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Intawat Nookaew

Chalmers, Biologi och bioteknik, Systembiologi

Britt Gabrielsson

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ann-Sofie Sandberg

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Nutrients

2072-6643 (ISSN)

Vol. 8 543- 543

Ämneskategorier

Biokemi och molekylärbiologi

Bioinformatik (beräkningsbiologi)

Bioinformatik och systembiologi

Infrastruktur

Chalmers infrastruktur för masspektrometri

Styrkeområden

Livsvetenskaper och teknik

DOI

10.3390/nu8090543