Eicosapentaenoic and Docosahexaenoic Acid-Enriched High Fat Diet Delays Skeletal Muscle Degradation in Mice
Journal article, 2016

Low-grade chronic inflammatory conditions such as ageing, obesity and related metabolic disorders are associated with deterioration of skeletal muscle (SkM). Human studies have shown that marine fatty acids influence SkM function, though the underlying mechanisms of action are unknown. As a model of diet-induced obesity, we fed C57BL/6J mice either a high fat diet (HFD) with purified marine fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (HFD-ED), a HFD with corn oil, or normal mouse chow for 8 weeks; and used transcriptomics to identify the molecular effects of EPA and DHA on SkM. Consumption of ED-enriched HFD modulated SkM metabolism through increased gene expression of mitochondrial β-oxidation and slow-fiber type genes compared with HFD-corn oil fed mice. Furthermore, HFD-ED intake increased nuclear localization of nuclear factor of activated T-cells (Nfatc4) protein, which controls fiber-type composition. This data suggests a role for EPA and DHA in mitigating some of the molecular responses due to a HFD in SkM. Overall, the results suggest that increased consumption of the marine fatty acids EPA and DHA may aid in the prevention of molecular processes that lead to muscle deterioration commonly associated with obesity-induced low-grade inflammation.

obesity

skeletal-muscle metabolism

transcriptome

mitochondrial β-oxidation

eicosapentaenoic acid (EPA)/Docosahexaenoic acid (DHA)

Author

Nikulkumar Soni

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Alastair Ross

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Nathalie Scheers

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Otto Savolainen

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Intawat Nookaew

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Britt Gabrielsson

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Ann-Sofie Sandberg

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Nutrients

2072-6643 (ISSN)

Vol. 8 9 543- 543

Subject Categories

Biochemistry and Molecular Biology

Bioinformatics (Computational Biology)

Bioinformatics and Systems Biology

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.3390/nu8090543

More information

Created

10/7/2017