The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues.
Artikel i vetenskaplig tidskrift, 2019

Excess energy intake can trigger an uncontrolled inflammatory response, leading to systemic low-grade inflammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are suggested to mitigate this inflammatory response, but the mechanisms are unclear, especially at the tissue level. Adipose tissues, the first tissues to give an inflammatory response, may be an important target site of action for EPA and DHA. To evaluate the effects of EPA and DHA in white and brown adipose tissues, we fed male C57Bl/6J mice either a high fat diet (HFD) with 5% corn oil, an HFD with 40% of the corn oil substituted for purified EPA and DHA triglycerides (HFD-ED), or normal chow, for 8 weeks. Fatty acid profiling and transcriptomics were used to study how EPA and DHA affect retroperitoneal white and brown adipose tissues. HFD-EDfed mice showed reduced lipid accumulation and levels of the pro-inflammatory fatty acid arachidonic acid in both white and brown adipose tissues, compared withHFD-cornoil fed animals. The transcriptomic analysis showed changes inβ-oxidation pathways, supporting the decreased lipid accumulation in the HFD-ED fed mice. Therefore, our data suggests that EPA and DHA supplementation of a high fat diet may be anti-inflammatory, as well as reduce lipid accumulation in adipose tissues.

brown and white adipose tissue

signaling pathway

gene expression profiling

inflammation

docosahexaenoic acid (DHA)

eicosapentaenoic acid (EPA)

Författare

Nikulkumar Soni

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Alastair Ross

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Intawat Nookaew

Chalmers, Biologi och bioteknik, Systembiologi

University of Arkansas for Medical Sciences

Nathalie Scheers

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Britt Gabrielsson

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ann-Sofie Sandberg

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

International Journal of Molecular Sciences

16616596 (ISSN) 14220067 (eISSN)

Vol. 20 23

Design av nya marina livsmedel för ett hälsosamt åldrande

Formas, 2012-01-01 -- 2015-12-31.

Västra Götalandsregionen, Regionutvecklingssekretariatet, 2012-01-01 -- 2015-06-30.

Ämneskategorier

Cell- och molekylärbiologi

Odontologi

Styrkeområden

Livsvetenskaper och teknik (2010-2018)

DOI

10.3390/ijms 20235895

PubMed

31771283

Mer information

Senast uppdaterat

2020-01-10