The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues.
Artikel i vetenskaplig tidskrift, 2019
Excess energy intake can trigger an uncontrolled inﬂammatory response, leading to systemic low-grade inﬂammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are suggested to mitigate this inﬂammatory response, but the mechanisms are unclear, especially at the tissue level. Adipose tissues, the ﬁrst tissues to give an inﬂammatory response, may be an important target site of action for EPA and DHA. To evaluate the eﬀects of EPA and DHA in white and brown adipose tissues, we fed male C57Bl/6J mice either a high fat diet (HFD) with 5% corn oil, an HFD with 40% of the corn oil substituted for puriﬁed EPA and DHA triglycerides (HFD-ED), or normal chow, for 8 weeks. Fatty acid proﬁling and transcriptomics were used to study how EPA and DHA aﬀect retroperitoneal white and brown adipose tissues. HFD-EDfed mice showed reduced lipid accumulation and levels of the pro-inﬂammatory fatty acid arachidonic acid in both white and brown adipose tissues, compared withHFD-cornoil fed animals. The transcriptomic analysis showed changes inβ-oxidation pathways, supporting the decreased lipid accumulation in the HFD-ED fed mice. Therefore, our data suggests that EPA and DHA supplementation of a high fat diet may be anti-inﬂammatory, as well as reduce lipid accumulation in adipose tissues.
brown and white adipose tissue
gene expression proﬁling
docosahexaenoic acid (DHA)
eicosapentaenoic acid (EPA)