John Fletcher

2021

Correlated fluorescence microscopy and multi-ion beam secondary ion mass spectrometry imaging reveals phosphatidylethanolamine increases in the membrane of cancer cells over-expressing the molecular chaperone subunit CCTδ

2020

Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations in Drosophila Brain Determined by Imaging Mass Spectrometry

Lipid Diversity in Cells and Tissue Using Imaging SIMS

Benefits of NaCl addition for time-of-flight secondary ion mass spectrometry analysis including the discrimination of diacylglyceride and triacylglyceride ions

Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in Drosophila Brain

On-Tissue Chemical Derivatization of Catecholamines Using 4-(N-Methyl)pyridinium Boronic Acid for ToF-SIMS and LDI-ToF Mass Spectrometry Imaging

2017

MS/MS analysis and imaging of lipids across Drosophila brain using secondary ion mass spectrometry

Multimodal Imaging of Chemically Fixed Cells in Preparation for NanoSIMS

Optimizing sample preparation for anatomical determination in the hippocampus of rodent brain by ToF-SIMS analysis

Peptide Fragmentation and Surface Structural Analysis by Means of ToF-SIMS Using Large Cluster Ion Sources

2016

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis

Evaluation of biomolecular distributions in rat brain tissues by means of ToF-SIMS using a continuous beam of Ar clusters

Investigating the Role of the Stringent Response in Lipid Modifications during the Stationary Phase in E. coli by Direct Analysis with Time-of-Flight-Secondary Ion Mass Spectrometry

Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry

High energy gas cluster ions for organic and biological analysis by time-of-flight secondary ion mass spectrometry

Comparing depth profiling of oxide scale on SOFC interconnect-materials using ToF-SIMS with Ga-69(+), Bi-3(+)/Cs+ and C-60(+)/C-60(2+) as primary and sputter ions

Lipid Structural Effects of Oral Administration of Methylphenidate in Drosophila Brain by Secondary Ion Mass Spectrometry Imaging

Latest applications of 3D ToF-SIMS bio-imaging

Measuring Compositions in Organic Depth Profiling: Results from a VAMAS Interlaboratory Study

Significant Enhancement of Negative Secondary Ion Yields by Cluster Ion Bombardment Combined with Cesium Flooding

3D imaging of biological specimen using MS

Improved Molecular Imaging in Rodent Brain with Time-of-Flight-Secondary Ion Mass Spectrometry Using Gas Cluster Ion Beams and Reactive Vapor Exposure

Prospect of increasing secondary ion yields in ToF-SIMS using water cluster primary ion beams

2014

Analysis of liposome model systems by time-of-flight secondary ion mass spectrometry

Gold and silver nanoparticle-assisted laser desorption ionization mass spectrometry compatible with secondary ion mass spectrometry for lipid analysis

Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) Imaging

Imaging Mass Spectrometry for Single-Cell Analysis

3D Imaging of TiO2 nanoparticle accumulation in tetrahymena pyriformis

Examination of fragment ions of polystyrene in TOF-SIMS spectra using MS/MS

ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain

Maximising the potential for bacterial phenotyping using time‐of‐flight secondary ion mass spectrometry with multivariate analysis and Tandem Mass Spectrometry

Evaluating the challenges associated with time-of-fight secondary ion mass spectrometry for metabolomics using pure and mixed metabolites

Compositional characterisation of metallurgical grade silicon and porous silicon nanosponge particles

ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Peptide structural analysis using continuous Ar cluster and C60 ion beams

Enhancing Secondary Ion Yields in Time of Flight-Secondary Ion Mass Spectrometry Using Water Cluster Primary Beams

Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer

Time-of-Flight Secondary Ion Mass Spectrometry Based Molecular Histology of Human Spinal Cord Tissue and Motor Neurons

Secondary Ion Mass Spectrometry: Characterizing Complex Samples in Two and Three Dimensions.

Comparison of C60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections

Peak picking as a pre-processing technique for imaging time of flight secondary ion mass spectrometry

Cluster and polyatomic primary ion beams

The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications.

A Comparative Study of Secondary Ion Emission from Water Ice under Ion Bombardment by Au(+), Au(3)(+), and C(60)(+)

TOF-SIMS with Argon Gas Cluster Ion Beams: A Comparison with C(60)(+)

Molecular SIMS imaging; spatial resolution and molecular sensitivity: Have we reached the end of the road? Is there light at the end of the tunnel?

Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation

Top-down approach to studying biological components using ToF-SIMS

DEVELOPMENTS IN MOLECULAR SIMS DEPTH PROFILING AND 3D IMAGING OF BIOLOGICAL SYSTEMS USING POLYATOMIC PRIMARY IONS

Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager

A new time-of-flight SIMS instrument for 3D imaging and analysis

Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.

Investigating the effect of temperature on depth profiles of biological material using ToF‐SIMS

Effects of Cryogenic Sample Analysis on Molecular Depth Profiles with TOF-Secondary Ion Mass Spectrometry

2 and 3D TOF-SIMS Imaging for Biological Analysis

Time of Flight Mass Spectrometry Imaging of Samples Fractured In Situ with a Spring-Loaded Trap System.

A new SIMS paradigm for 2D and 3D molecular imaging of bio-systems

A Comparative Study of Secondary Ion Emission from Water Ice under Ion Bombardment by Au+, Au3+, and C60+

Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates

Cellular imaging with secondary ion mass spectrometry

A comparison of PCA and MAF for ToF-SIMS image interpretation

Uncovering new challenges in bio-analysis with ToF-SIMS

A New Dynamic in Mass Spectral Imaging of Single Biological Cells

Substrate effects on the analysis of biomolecular layers using Au+, Au3+ and C60+ bombardments

Subsurface Biomolecular Imaging of Streptomyces coelicolor Using Secondary Ion Mass Spectrometry

TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium

Exploratory analysis of TOF-SIMS data from biological surfaces

Properties of C-84 and C24H12 molecular ion sources for routine TOF-SIMS analysis

TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C-60) primary ions

TOF-SIMS analysis using C-60- effect of impact energy on yield and damage

ToF-SIMS analysis of bio-systems: Are polyatomic primary ions the solution?

Molecular depth profiling of organic and biological materials

ToF-SIMS studies of Bacillus using multivariate analysis with possible identification and taxonomic applications

C-60, buckminsterfullerene: its impact on biological ToF-SIMS analysis

Rapid discrimination of the causal agents of urinary tract infection using ToF-SIMS with chemometric cluster analysis

ToF-SIMS studies of Bacillus using multivariate analysis with possible identification and taxonomic applications

A comparative study of secondary ion yield from model biological membranes using Au-n(+) and C-60(+) primary ion sources

ToF-SIMS Studies of Sulfuric Acid Hydrate Films

ToF-SIMS analysis of atmospherically relevant sulphuric acid hydrate films and reactions thereof

Identification of surface molecular hydrates on solid sulfuric acid films

First detection of molecular hydrate complexes in sulfuric acid aerosols

The role of surface molecular hydrates in the heterogeneous interaction of NH3 with sulfuric acid monohydrate